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ecis 16 well array station  (Applied BioPhysics)
96
Applied BioPhysics ecis 16 well array station
Mock-, HTNV-, or ANDV-infected HUVEC were trypsinized, seeded onto <t>ECIS</t> chamberslides, and cultured until confluent. Media were removed and replaced with phenol red free EBM containing Zn 2+ . After an equilibration, 0.2 ml of phenol red free EBM containing Zn 2+ , FXII, PK, and HK (100 nM each) were added to cells (time zero) (black lines) (A). To measure inhibition of activation, some samples were treated with CTI (1 µM) (blue line), PKSI-527 (5 µM) (red line), or HOE 140 (1 µM) (green line) simultaneously with factors (B, C, & D). Real time measurements frequency measurements were taken throughout the assay.
Ecis 16 Well Array Station, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/16w+array+station/pmc03715459-251-6-16?v=Applied+BioPhysics
Average 96 stars, based on 1 article reviews
ecis 16 well array station - by Bioz Stars, 2026-08
96/100 stars
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Mock-, HTNV-, or ANDV-infected HUVEC were trypsinized, seeded onto ECIS chamberslides, and cultured until confluent. Media were removed and replaced with phenol red free EBM containing Zn 2+ . After an equilibration, 0.2 ml of phenol red free EBM containing Zn 2+ , FXII, PK, and HK (100 nM each) were added to cells (time zero) (black lines) (A). To measure inhibition of activation, some samples were treated with CTI (1 µM) (blue line), PKSI-527 (5 µM) (red line), or HOE 140 (1 µM) (green line) simultaneously with factors (B, C, & D). Real time measurements frequency measurements were taken throughout the assay.

Journal: PLoS Pathogens

Article Title: Endothelial Cell Permeability during Hantavirus Infection Involves Factor XII-Dependent Increased Activation of the Kallikrein-Kinin System

doi: 10.1371/journal.ppat.1003470

Figure Lengend Snippet: Mock-, HTNV-, or ANDV-infected HUVEC were trypsinized, seeded onto ECIS chamberslides, and cultured until confluent. Media were removed and replaced with phenol red free EBM containing Zn 2+ . After an equilibration, 0.2 ml of phenol red free EBM containing Zn 2+ , FXII, PK, and HK (100 nM each) were added to cells (time zero) (black lines) (A). To measure inhibition of activation, some samples were treated with CTI (1 µM) (blue line), PKSI-527 (5 µM) (red line), or HOE 140 (1 µM) (green line) simultaneously with factors (B, C, & D). Real time measurements frequency measurements were taken throughout the assay.

Article Snippet: Arrays were then loaded into the ECIS 16-well array station and stabilized using ECIS software v1.2.92.0 (Applied Biophysics).

Techniques: Infection, Cell Culture, Inhibition, Activation Assay